Dr. Syed Qaswar Ali Shah (Head of Department)

PhD (Norwegian School Of Veterinary Sciences Norway), Postdoc


Mobile: 0304-72-688-31  
Email: syedqaswaralishah@cuvas.edu.pk

Biography:

Dr. Syed Qaswar Ali Shah did Master in Zoology from the Bahauddin Zakariya University Multan and was awarded Gold Medal in 1994. In 1995, he was appointed as a part time Lecturer in Zoology at the Bahauddin Zakariya University Multan and in 1996, he joined the Punjab Higher Education Department as a Lecturer in Biology after selection by the Punjab Public Service Commission. He did M.Phil. in Zoology in 2005 from the Bahauddin Zakariya University Multan.
In 2007, he was awarded Scholarship for PhD under “Overseas Scholarship Phase 1, Batch 2” scheme and completed his PhD from the Norwegian School of Veterinary Science (2008 – 2012) entitled “Antimicrobial resistance in fish pathogenic and aquatic environmental bacteria”.
During his doctoral study he has been involved in all processes of bacterial sampling, cultivation, drug resistance testing and laboratory techniques related to DNA isolation, DNA-DNA hybridization, conjugation, cloning, PCR-amplification, qPCR. He also got experience in using various microbiological laboratory techniques and instruments particularly related to drug resistance testing and dissemination of resistance among bacterial pathogenic and environmental bacteria. He used various bioinformatics and genetic tools to analyze the results. He sequenced and submitted various gene sequences in GenBank data which are available online.
From 2014 – 2016, he worked on a Postdoc project related to the “Prevention of bacterial infections and control of antibiotic resistance in the food chainhttp://uc-care.ku.dk/english/ “ in the Department of Veterinary Disease Biology, University of Copenhagen, Denmark. In 2016, he joined the Punjab Higher Education Department as an Associate Professor of Zoology and served till 2018. In 2019, he joined the Cholistan University of Veterinary and Animal Sciences and is serving as an Associate Professor of Zoology.

Areas of Interest

Zoology, Biological Sciences, Fisheries, Aquaculture, Microbiology, Bacteriology, Epidemiology, Veterinary Medicines, Infectious diseases, Antibiotic resistance

Work Experience:

March 2014 -
Feb 2016

Postdoc Researcher

Department of Veterinary Disease Biology, University of Copenhagen          

  • Project: Prevention of bacterial infections and control of antibiotic resistance in the food chain  http://uc-care.ku.dk/english/
  • Worked on a Pproject related to screening, differentiation and quantification of different extended spectrum β-lactamase [ESBL] genes by Multiplex Real-time PCR (qPCR) occurring in poultry products and hatchery samples.

Aug 2008 –
Dec 2012

PhD Researcher

Department of Food Safety and Infection Biology Norwegian School of Veterinary Sciences

  • PhD Project: Antibiotic resistance in fish pathogenic and aquatic/sedimental environmental bacteria
  • Principal objective of the study was to explore the genetic mechanisms involved in development of antibiotic resistance in pathogenic and aquatic environmental bacteria. .

Jan 2002 –
Dec 2004

Bahauddin Zakariya University Multan, Pakistan                                                

Project: Stress responses in fish

  • Principal objective was to determine effects of starvation and Ph stress on the growth and body composition of a fish (Labeo rohita)

 

Education and Training:

Aug 2008 –
Dec 2012

PhD Microbiology (Veterinary Medicine and Science)

Department of Food Safety and Infection Biology, Norwegian School of Veterinary Sciences

Jan 2002 –
Dec 2004

Master of Philosophy (M Phil)  in Zoology

  • Bahauddin Zakariya University Multan, Pakistan                                                

Mar 1996 –
Oct 1997

Master of Education (M Ed) in Biology and Chemistry

  • Bahauddin Zakariya University Multan, Pakistan                                                

Technical and Laboratory Skills:

Molecular Biology

  • Media plates and buffers preparations,
  • Isolation, cultivation and identification of pathogenic and environmental bacteria, CFU determination.
  •  Antibiotic susceptibility testing by disk diffusion, MIC,
  • Screening of resistance genes and plasmids by DNA-DNA hybridization and PCR,
  • Cloning of genes in plasmids and chromosomes
  • Conjugation experiments

DNA techniques

 

  • R plasmid isolation, DNA/RNA isolation, cDNA synthesis, designing/validation of probes and primers
  • PCR-amplification and agrose gel electrophoresis of DNA,
  • MLSA, RFLP, MALDI-TOF
  • RT-qPCR by Syber green and Taqman probe based assay,  absolute /relative quantification,

Bioinformatics

  • Moderate knowledge of various bioinformatics tools like Artemis, NCBI BLAST, Seqin, MEGA and use of various softwares.

Publications:

  • Hussain M. Z., Qasim, M., Ali, A., Shah, S.Q.A., Aziz, S. and Mustafa G. 2017. Breeding rate and mortality in Australian parrot (Melopsittacus undulatus) in captivity at Bahawal Pur, Pakistan Pure Appl. Biol.  http://dx.doi.org/10.19045/bspab.2017.60051
  • Shah, S. Q. A., Hussain, M. Z., Ali, M., Salam, A. 2017. Effect of Stress Conditions on Body Composition Parameters of Farmed Rohu (Labeo rohita). Turk. J. Fish. Aquat. Sci. 17: 471-476. doi: 10.4194/1303-2712-v17_3_03
  • Espinosa-Gongora C1, Shah S. Q1, Jessen LR1, Bortolaia V., Langebæk R., Bjørnvad C. R., Guardabassi L. 2015. Quantitative assessment of faecal shedding of β-lactam-resistant Escherichia coli and enterococci in dogs. Vet. Microbiol. 181(3-4):298-302.   doi: 10.1016/j.vetmic.2015.10.004. 1The first three authors contributed equally to this work.
  • Hussain, M. Z. and Shah S.Q.A. 2016. Effect of stress conditions on condition factor and growth of farmed Rohu (Labeo rohita) J. Entomol. Zool. Stud. 4(5):258-261.
  • Shah, S. Q. A., T. M. L’Abée-Lund, A. Tomova, A. H. Buschmann, F. C. Cabello, H. Sørum. 2014. Antimicrobial resistance and antimicrobial resistance genes in marine bacteria from a salmon aquaculture and non-aquaculture sites. Environ Microbiol. 16(5): 1310–1320 doi: 10.1111/1462-2920.12421
  • Shah, S. Q. A. and H. Sørum. 2014. Genetic localization of a TetR-like transcriptional regulator gene in Pseudomonas fluorescens isolated from farmed fish. J Appl. Genet. doi: 10.1007/s13353-014-0221-1
  • Cantas, L., S. Q. A. Shah, L. M. Cavaco, C. Manaia, F. Walsh, M. Popowska, H. Garelick, H. Bnrgmann, and H. Sørum. 2013. A brief multi-disciplinary review on antimicrobial resistance in medicine and its linkage to the global environmental microbiota. Front. Microbiol. 4. doi:10.3389/fmicb.2013.00096.
  • Shah, S. Q. A., D. J. Colquhoun, Hamisi L. Nikuli , H. Sørum .2012.  Prevalence of antibiotic resistance genes in the bacterial flora of integrated fish farming environments of Pakistan and Tanzania. J. Env. Sci. Technol. 46: 8672-8679. doi: 10.1021/es3018607
  • Shah, S. Q. A., H. Nilsen, K. Bottolfsen, D. J. Colquhoun, and H. Sørum. 2012.  DNA gyrase and topoisomerase IV mutations in quinolone resistant Flavobacterium psychrophilum isolated from diseased salmonids in Norway. Microb. Drug Resist. 18(2): 207-214. doi:10.1089/mdr.2011.0142.
  • Shah, S. Q. A., S. Karatas, H. Nilsen, T. M. Steinium, D. J. Colquhoun, and H. Sørum. 2012. Characterization and expression of the gyrA gene from quinolone resistant Yersinia ruckeri strains isolated from Atlantic salmon (Salmo salar L.). Aquaculture. 350-353: 37-41. doi: http://doi.org/10.1016/j.aquaculture.2012.04.022
  • Gilbert, M., M. Z. Virani, R.T. Watson, J. L. Oaks, P. C. Benson, A. A. Khan, S. Ahmad, J. Chaudry, M. Arshad, S. Mahmood, Q. A. Shah. 2002. Breeding and mortality of Oriental White backed Vulture Gyps bengalensis in Punjab Province, Pakistan. Bird Conserv. Int 12:311-326. doi: https://doi.org/10.1017/S0959270902002198
  • Virani, M., R. Watson, I. Oaks, M. Gilbert, P. Benson, A. A. Khan, H. S. Baral, Q. A. Shah, J.B. Girl. 2001. The Asian Vultures Crisis ---- a global perspective. In L. Bennun and M. Virani, eds. Responding to the Asian Vulture crisis: Planning for Vulture monitoring and conservation in Kenya, Proceedings and recommendations of a seminar and workshop held at the National Museums of Kenya, May 2001. NMK Ornithol.Res.Report 41, June 2001.

Awards and honours:

  • Gold Medal in MSc. Zoology
  • Granted Overseas PhD Scholarship from HEC, Pakistan
  • Reviewer for various peer reviewed international journals related to life sciences including Chemosphere, Applied and Environmental Microbiology, Aquaculture, Journal of Toxicology and Environmental Health, Pakistan journal of Zoology
  • PhD research articles related to fish pathogens were part of Norwegian Fish Health report 2011 published by the Norwegian Veterinary Institute http://www.vetinst.no/eng/Publications/Fish-Health-Report

Conferences Attended:

  • Shah S Q A., Asma, Bibi, S. " Prevalence and antibiotic susceptibility profile of Escherichia coli O157:H7 in water and raw milk " Oral presentation (4th International Conference on “Food and Nutrition Security in changing climate” organized by Institute of Food Science and Nutrition BZ University Multan) 3 – 4 March 2019.
  • Shah S Q A. "Prevalence of antibiotic resistance in aquatic environmental bacteria of fish farming facilities in Pakistan" Oral presentation (11th International Conference jointly organized by Pakistan Society of Microbiology, American Society of Microbiology & Institute of Food Science and Nutrition BZ University Multan) 18 – 20 Dec. 2017.
  • Valeria Bortolaia, V, Shah S Q A, Jensen H U, Christensen J P, Christensen H, Guardabassi L. "Low prevalence of cephalosporin-resistant Escherichia coli in Danish broiler production, 2014-2015" Poster presentation ICOHAR (International Conference on One Health Antimicrobial Resistance) 2015. Copenhagen September 30 – October 2.
  • Shah S Q A and Sørum H ―Use of prebiotics and probiotics: An alternative to antimicrobials in integrated fish farming‖. Oral and Poster presentation Keele University Manchester (Prebiotics and Probiotics in Medicine, Veterinary Sciences and Aquaculture September 2012).
  • Shah S Q A, Colquhoun D, Cabello F and Sørum H ― Antimicrobial resistance in aquatic environmental bacteria associated with fish farms and pond sediments‖. Poster presentation SETAC (Society of Environmental Toxicology and Chemistry) World Congress Berlin 2012
  •  Shah S Q A and Sørum H "Antibiotic resistance among aquatic sedimental bacteria of freshwater fish farming environments". Oral presentation ARAE (Antimicrobial Resistance in Animals and the Environment) 2011. Tours, France 27 June 2011.
  • Shah S Q A and Sørum H "Quinolone resistance in Flavobacterium psychrophilum isolated from diseased salmonids in Norway". Oral presentation — Communication Network in Marine Bacteria) Tromsø June 2011
  • Shah S Q A, Cantas L and Sørum H "Antibiotic resistance in aquatic environmental bacteria". Henning Zurich — 24 Nov. 2010. Poster presentation. COST ACTION (European Cooperation in Science and Technology).
  • Shah S Q A and Sørum H ―gyrA mutations in quinolone resistant Flavobacterium psychrophilum from a disease outbreak in famed rainbow trout on the west coast of Norway. June 2009. Poster presentation in FEMS 2009 — 3rd congress of European Microbiologists Gothenberg, Sweden.

Memberships

  • SETAC (Society of Environmental Toxicology and Chemistry)
  • DARE COST ACTION (European Cooperation in Science and Technology)
  • Norsk forening for Mikrobiologi/FEMS (Federation of European Microbiological Societies)